primary antibodies against toll Search Results


biotinylated secondary antibody against mouse  (Vector Laboratories)
96
Vector Laboratories biotinylated secondary antibody against mouse
Biotinylated Secondary Antibody Against Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated secondary antibody against mouse - by Bioz Stars, 2026-02
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antibodies against pkc alpha  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology antibodies against pkc alpha
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Antibodies Against Pkc Alpha, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against pkc alpha - by Bioz Stars, 2026-02
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monoclonal mouse antibody against p16ink4a  (Thermo Fisher)
90
Thermo Fisher monoclonal mouse antibody against p16ink4a
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Monoclonal Mouse Antibody Against P16ink4a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against egfr  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology primary antibodies against egfr
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Antibodies Against Egfr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the primary antibody against actin  (Millipore)
90
Millipore the primary antibody against actin
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
The Primary Antibody Against Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the primary antibody against actin - by Bioz Stars, 2026-02
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primary antibody against β-actin  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology primary antibody against β-actin
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Antibody Against β Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody against β-actin - by Bioz Stars, 2026-02
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primary antibodies against caspase-8  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc primary antibodies against caspase-8
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Antibodies Against Caspase 8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal antibody against ul44  (Biosynth Carbosynth)
86
Biosynth Carbosynth mouse monoclonal antibody against ul44
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Mouse Monoclonal Antibody Against Ul44, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody against cx40  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology primary antibody against cx40
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Antibody Against Cx40, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary anti cd14  (Boster Bio)
90
Boster Bio primary anti cd14
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Anti Cd14, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against human chromogranin  (Agilent technologies)
90
Agilent technologies primary antibodies against human chromogranin
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Antibodies Against Human Chromogranin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against pedf  (Millipore)
90
Millipore primary antibodies against pedf
Fig. 3. Evaluation of the role of individual <t>PKC</t> isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC <t>alpha,</t> delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.
Primary Antibodies Against Pedf, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. Evaluation of the role of individual PKC isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC alpha, delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.

Journal: Biochemical pharmacology

Article Title: The synthetic bryostatin analog Merle 23 dissects distinct mechanisms of bryostatin activity in the LNCaP human prostate cancer cell line.

doi: 10.1016/j.bcp.2011.03.018

Figure Lengend Snippet: Fig. 3. Evaluation of the role of individual PKC isoforms in the biological effects of PMA, bryostatin 1 and Merle 23 using PKC inhibitors or siRNA. (A) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on cell growth in the presence of vehicle (DMSO) or 100 nM PMA, bryostatin 1 or Merle 23 was determined by the Incucyte as described for Fig. 2A. Values represent the mean S.E.M. of thtee independent experiments. PMA versus PMA + Go¨6983, p = 0.0091; PMA versus PMA + Go¨6976, p = 0.44. (B) The effect of different siRNAs on cell growth. Cells were transfected with the indicated siRNAs as described in Section 2 and were treated 48 h later with DMSO as control or 300 nM PMA, bryostatin 1 or Merle 23 for 48–60 h. Confluency was determined by the Incucyte. Values represent the mean S.E.M. of seven independent experiments. Co = control, non-treated cells; scr = scrambled siRNA; a, d, e si = siRNA against PKC alpha, delta and epsilon isoforms, respectively. p values for Merle 23 control versus DMSO, PMA and bryostatin1 control are 0.0015, 0.0046 and 0.010, respectively; p values for delta siRNA versus control for PMA and Merle 23 are p < 0.0001 and 0.014; all p values for PMA and Merle 23 between control and alpha or epsilon siRNAs are >0.5. (C) The effect of PKC inhibitors Go¨6976 (2 mM) and Go¨6983 (2 mM) on TNF-alpha secretion induced by 10 nM PMA, bryostatin 1 or Merle 23. Secretion of TNF-alpha into the supernatants was measured by ELISA 24 h after treatment. Values represent the mean S.E.M. of three independent experiments. PMA versus PMA + Go¨6976, p = 0.0005. (D) The effect of different siRNAs on TNF-alpha secretion. Cells transfected with the indicated siRNAs as described in Section 2 were treated 48 h later with DMSO as control or 10 nM PMA, 100 nM bryostatin 1 or 100 nM Merle 23 for 24 h. TNF-alpha secreted into the supernatant was measured by ELISA. Values represent the mean S.E.M. of three independent experiments. For abbreviations see legend above. PMA versus PMA + alpha siRNA, p = 0.0006.

Article Snippet: The primary antibodies against PKC alpha (C-20), delta (C-20), epsilon (C-15), beta (C-16 and C-18), eta (31), theta (C-18 and 1C2), p65 (F-6), and cFos (H-125) were from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Transfection, Control, Enzyme-linked Immunosorbent Assay